Table 1. Different methods used for extraction of metagenomic DNA from four different soils.

Method	Homogenizing agent	Homogenizing equipment	Processing time (h)	DNA Yield(μg/g of soil)
M1 [39]	Extraction buffer	Nil	5	1.29 ± 0.02
M2 [6]	Glass beads	Bead beater	7	3.42 ± 0.04
M3 [6]	Nil	Sonicator	7	1.47 ± 0.04
M4 [10]	Lysozyme	-70°C deep freezer and dryice ethanol bath	8	3.38 ± 0.05
M5 (Fast DNA SPIN kit)	As per the manufacturer’s protocol	Bead beater	1.5	3.51 ± 0.03
M6 (present study)	Glass Powder	Nil	1.5	5.87 ± 0.04

DNA yield represents the Average ± SD values of four different soils.

Nil represents there is no usage of homogenizing equipment.