Table 4. Assessment of purity of metagenomic DNA by methods M1 to M6 for different soils.
A260/280 | A340 | |||||||
---|---|---|---|---|---|---|---|---|
Methods | Garden soil | Sewage sludge | Lake soil | Compost | Garden soil | Sewage sludge | Lake soil | Compost |
M1 | 1.14 ± 0.02 | 1.22 ± 0.03 | 1.22 ± 0.09 | 1.29 ± 0.04 | 0.09 ± 0.03 | 0.08 ± 0.02 | 0.09 ± 0.03 | 0.08 ± 0.01 |
M2 | 1.53 ± 0.06 | 1.48 ± 0.03 | 1.56 ± 0.01 | 1.40 ± 0.07 | 0.06 ± 0.02 | 0.07 ± 0.03 | 0.08 ± 0.02 | 0.06 ± 0.04 |
M3 | 1.32 ± 0.03 | 1.25 ± 0.05 | 1.34 ± 0.01 | 1.37 ± 0.06 | 0.07 ± 0.01 | 0.08 ± 0.03 | 0.06 ± 0.04 | 0.06 ± 0.05 |
M4 | 1.33 ± 0.05 | 1.34 ± 0.06 | 1.46 ± 0.09 | 1.48 ± 0.03 | 0.09 ± 0.04 | 0.08 ± 0.02 | 0.08 ± 0.03 | 0.09 ± 0.02 |
M5 | 1.60 ± 0.04 | 1.62 ± 0.08 | 1.56 ± 0.03 | 1.50 ± 0.07 | 0.06 ± 0.04 | 0.06 ± 0.03 | 0.07 ± 0.08 | 0.08 ± 0.01 |
M6 | 1.82 ± 0.08 | 1.72 ± 0.04 | 1.78 ± 0.02 | 1.73 ± 0.05 | 0.04 ± 0.03 | 0.06 ± 0.04 | 0.05 ± 0.01 | 0.04 ± 0.06 |
A260/280 represents protein contamination. A340 is an indication for humic acid presence.