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. 2015 Jul 13;10(7):e0132377. doi: 10.1371/journal.pone.0132377

Fig 2. Dependence of tumorsphere growth on culture medium in 2D versus 3D for MDA231 cells.

Fig 2

Number density (A) and mRNA expression of CD44 (B), ABCG2 (C), and EGFR (D) markers for MDA231 cells as a function of incubation time. Groups included cells on 2D adherent plates and cultured in RPMI-1640 medium (2D-RPMI), cells on 2D plates and cultured in CSC medium (2D-CSC), and cells encapsulated in the 5 kPa PEGDA gel and cultured in CSC medium (3D-CSC). An Asterisk in (A) indicates a statistically lower (p<0.05) cell number in the test group compared to 2D groups at the same time point. An Asterisk in (B-D) indicates a statistically higher (p<0.05) mRNA expression in the test group compared to 2D groups at the same time point. The p-values for the asterisks in (A-D) are listed in Tables E-H in S1 File. Error bars correspond to means±1 SD for n = 3.