mRNA expression of EMT markers E-cadherin (A), N-cadherin (B), TGF-β (C), Snail (D), Slug (E), Twist (F), Vimentin (G), and ZEB2 (H) for MDA231 cells encapsulated in the un-patterned 5 kPa gel as a function of incubation time. Protein expression of pYAP (I), total YAP (J), YAP/TAZ (K) for the MDA231 cells encapsualted in the un-patterned gel as a function of modulus after 6 days of incubation. An asterisk in (A) indicates a statistically lower marker expression for that time point compared to all other time points. An asterisk in (B,D-H) indicates a statistically higher marker expression for that time point compared to all other time points. An asterisk in (I) indicates a statistically lower protein expression for that modulus compared to the other two moduli. An asterisk in (k) indicates a statistically higher protein expression for that modulus compared to the other two moduli. The p-values for the asterisks in (A,B,D-I,K) are listed in Tables G-O in S2 File. Error bars correspond to mean±1 SD for n = 3.