Table 1.
Microarray analysis of expression regulation of genes downstream of the Wnt/β-catenin pathway upon 20 μM FH535 treatment
| Gene | ID | Normalized intensity
|
|
|---|---|---|---|
| Control | FH535 | ||
| MYC | 4609 | 16.268158 | 15.204586 |
| KRT18 | 3875 | 15.975001 | 16.022995 |
| PTTG1 | 9232 | 15.680945 | 15.73604 |
| ANGPTL4 | 51129 | 15.190848 | 15.278334 |
| KRT81 | 3887 | 15.0413 | 14.423697 |
| CD44 | 960 | 15.006962 | 16.199093 |
| PFDN5 | 5204 | 14.879261 | 14.964103 |
| KRT10 | 3858 | 14.799751 | 13.889791 |
| PTTG2 | 10744 | 14.772796 | 14.547727 |
| BIRC5 | 332 | 14.757564 | 14.219355 |
| PTTG1IP | 754 | 14.684395 | 14.533192 |
| VEGFB | 7423 | 14.498004 | 13.671163 |
| CYR61 | 3491 | 14.279853 | 14.790296 |
| UBXN1 | 51035 | 14.231482 | 14.049252 |
| KRT7 | 3855 | 14.184294 | 13.285099 |
| WISP2 | 8839 | 13.732449 | 12.493675 |
| SOX9 | 6662 | 13.574989 | 13.415171 |
| EN2 | 2020 | 13.393019 | 12.721889 |
| JAG1 | 182 | 12.427784 | 12.687155 |
| FOSL1 | 8061 | 12.344017 | 11.102832 |
| MYCBP | 26292 | 12.284651 | 11.974781 |
| SNAI1 | 6615 | 12.28132 | 10.385736 |
| KRT73 | 319101 | 12.23975 | 11.053284 |
| GJA1 | 2697 | 12.226766 | 13.521647 |
| IRX3 | 79191 | 12.224495 | 12.16053 |
| TBX1 | 6899 | 12.181493 | 12.043698 |
| DKK3 | 27122 | 12.076692 | 10.961267 |
| JUN | 3725 | 12.038464 | 12.673436 |
| MSL1 | 339287 | 11.920114 | 11.438548 |
| KRT80 | 144501 | 11.87818 | 12.039767 |
| CCND3 | 896 | 11.576098 | 10.075832 |
| CDKN2A | 1029 | 11.343829 | 11.097562 |
| EFNB1 | 1947 | 11.337793 | 10.368351 |
| PTTG3P | 26255 | 11.33311 | 10.750982 |
| KRT83 | 3889 | 11.319811 | 9.89329 |
| KRT19 | 3880 | 11.289505 | 11.101922 |
| CEBPD | 1052 | 11.196305 | 10.068165 |
| PPARD | 5467 | 11.19087 | 10.731722 |
| ANTXR1 | 84168 | 11.149265 | 12.122571 |
| EGFR | 1956 | 11.122326 | 12.333595 |
| CDX4 | 1046 | 10.933424 | 9.588729 |
| ISLR | 3671 | 10.854443 | 9.725897 |
| TWIST2 | 117581 | 10.853075 | 10.129753 |
| VEGFA | 7422 | 10.833399 | 10.087871 |
| CTGF | 1490 | 10.809845 | 11.98555 |
| FZD7 | 8324 | 10.711324 | 9.901575 |
| KRT85 | 3891 | 10.621079 | 9.606193 |
| CCND1 | 595 | 10.543621 | 10.14267 |
| TLE1 | 7088 | 10.343489 | 9.271956 |
| CDX1 | 1044 | 10.329419 | 9.136879 |
| KRT8 | 3856 | 10.267347 | 8.319682 |
| NRP1 | 8829 | 10.246916 | 9.627893 |
| DKK1 | 22943 | 10.211538 | 10.979951 |
| IRS1 | 3667 | 10.175792 | 10.3609915 |
| MMP2 | 4313 | 10.153262 | 9.412593 |
| IKBKG | 8517 | 10.132635 | 9.295626 |
| TIAM1 | 7074 | 10.117085 | 11.627998 |
| EGR1 | 1958 | 10.007974 | 8.180263 |
| MET | 4233 | 9.986441 | 12.74971 |
| BGLAP | 632 | 9.971276 | 9.414629 |
| VEGFC | 7424 | 9.923567 | 10.21814 |
| AHR | 196 | 9.886938 | 11.936481 |
| CACNA1G | 8913 | 9.812038 | 8.560494 |
| KRT15 | 3866 | 9.7214575 | 8.709181 |
| PPAP2B | 8613 | 9.718731 | 9.956581 |
| KRT86 | 3892 | 9.707824 | 9.012362 |
| KRT23 | 25984 | 9.573925 | 7.977122 |
| GBX2 | 2637 | 9.409858 | 9.626151 |
| KRT39 | 390792 | 9.284486 | 7.929165 |
| WNT3A | 89780 | 9.275467 | 8.68014 |
| PLAUR | 5329 | 9.265003 | 8.37788 |
| ID2 | 3398 | 9.226584 | 8.999163 |
| MAEA | 10296 | 9.087043 | 8.91366 |
| DAB2 | 1601 | 9.034534 | 10.517419 |
| ETS2 | 2114 | 8.999426 | 10.445461 |
| KRT31 | 3881 | 8.998071 | 7.96933 |
| TNFRSF11A | 8792 | 8.943393 | 9.029845 |
| RET | 5979 | 8.9224615 | 7.809108 |
| UBXN6 | 80700 | 8.850218 | 7.6966906 |
| STRA6 | 64220 | 8.746183 | 7.1663184 |
| KLF5 | 688 | 8.6543455 | 8.714795 |
| KRT4 | 3851 | 8.640165 | 7.6698284 |
| UBXN4 | 23190 | 8.607909 | 10.505396 |
| LEF1 | 51176 | 8.601926 | 9.380911 |
| KRT76 | 51350 | 8.571253 | 7.397269 |
| UBXN2B | 137886 | 8.2286415 | 9.982969 |
| UBXN11 | 91544 | 8.179481 | 7.272692 |
| LRP1 | 4035 | 8.175423 | 6.988433 |
| UBXN2A | 165324 | 8.133479 | 7.9562063 |
| KRT9 | 3857 | 8.110517 | 7.0731263 |
| EDA | 1896 | 8.09645 | 7.4072337 |
| KRT32 | 3882 | 8.087603 | 7.7537346 |
| FGF4 | 2249 | 7.9492774 | 6.5977035 |
| KRT3 | 3850 | 7.8908534 | 8.469248 |
| UBXN8 | 7993 | 7.86574 | 9.013798 |
| SIX1 | 6495 | 7.818405 | 7.9264607 |
| FOXN1 | 8456 | 7.7998743 | 6.8640747 |
| ETV6 | 2120 | 7.7085342 | 7.0067773 |
| KRT1 | 3848 | 7.5221066 | 6.7497764 |
| IL8 | 3576 | 7.501872 | 6.6113296 |
| NTRK2 | 4915 | 7.497469 | 7.1365094 |
| RUNX2 | 860 | 7.4688272 | 8.628798 |
| MMP11 | 4320 | 7.460847 | 7.2920337 |
| CDH1 | 999 | 7.3595057 | 7.319695 |
| TCF7L2 | 6934 | 7.3556123 | 8.6040535 |
| KRT78 | 196374 | 7.349466 | 6.8676143 |
| TCF7 | 6932 | 7.270456 | 7.664296 |
| SMO | 6608 | 7.222788 | 7.0400887 |
| EFNB2 | 1948 | 7.1960526 | 7.26771 |
| CLDN1 | 9076 | 7.1643777 | 8.943991 |
| KRT33A | 3883 | 7.121948 | 6.808277 |
| VCAN | 1462 | 7.045421 | 6.763195 |
| MMP9 | 4318 | 7.0101504 | 6.7540355 |
| DLL1 | 28514 | 6.969655 | 6.5782347 |
| KRT13 | 3860 | 6.949356 | 5.971072 |
| IGF2 | 3481 | 6.933426 | 6.170534 |
| KRT26 | 353288 | 6.869997 | 6.697632 |
| TNFRSF9 | 3604 | 6.862919 | 6.6031585 |
| KRT74 | 121391 | 6.778076 | 6.538765 |
| TWIST1 | 7291 | 6.765423 | 6.105777 |
| NRCAM | 4897 | 6.677019 | 6.7818675 |
| FGF9 | 2254 | 6.6647215 | 5.7855196 |
| TNFRSF11B | 4982 | 6.6092443 | 6.618697 |
| CHL1 | 10752 | 6.6082654 | 6.3569694 |
| KRT34 | 3885 | 6.601664 | 6.199431 |
| KRT6A | 3853 | 6.536037 | 5.9656916 |
| EDN1 | 1906 | 6.476451 | 6.7537594 |
| NOS2 | 4843 | 6.425461 | 6.333558 |
| GDF5 | 8200 | 6.3569694 | 6.3291264 |
| CCND2 | 894 | 6.3239446 | 5.996339 |
| DLK1 | 8788 | 6.2332454 | 6.884508 |
| KRT37 | 8688 | 5.971611 | 5.881136 |
| IL6 | 3569 | 5.7313643 | 5.9466343 |
| SOX2 | 6657 | 5.6166873 | 6.7975965 |
| TGFB3 | 7043 | 5.5891886 | 6.0552535 |
| KRT35 | 3886 | 5.5883365 | 6.3580856 |
| PTGS2 | 5743 | 5.5262737 | 7.601541 |
| BTRC | 8945 | 5.3152456 | 7.7473273 |