Figure 1.

Ubiquitin protein D (UBD) overexpression enhanced the resistance of epirubicin in triple-negative breast cancer cell lines. (A) Representative Western blot showing the overexpression of UBD in the MDA-MB-231 cell line transfected by UBD plasmid. (B) The inhibition rate of MDA-MB-231 cells treated with different concentrations of epirubicin by MTT, and the half maximal inhibitory concentration of MDA-MB-231 is about 20 nM. (C) The relative proliferation of UBD and its control cells was evaluated by MTT assay in MDA-MB-231 cell lines overexpressing UBD and their control plasmids treated with epirubicin (20 nM). The relative proliferation of blank triple-negative breast cancer cells, which were not treated with a chemotherapy drug, was also measured by MTT. (D) Migration assays were undertaken in MDA-MB-231 cells overexpressing UBD and their control plasmids treated with epirubicin (20 nM).

Notes: The lines above the bars indicated Standard Deviation. The asterisk (*) indicated P<0.05. The length of the scale bar indicated 100 μm.

Abbreviations: DMSO, dimethyl sulfoxide; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.