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. Author manuscript; available in PMC: 2015 Jul 13.
Published in final edited form as: Immunol Cell Biol. 2014 Aug 12;93(1):67–76. doi: 10.1038/icb.2014.68

Figure 5.

Figure 5

Representative ex vivo antigen-specific responses of KIR3DL1+CD8+ T cells from HLA-Bw6 subjects and representative ex vivo response to P815/anti-CD3 stimulation. Freshly-isolated PBMC from three KIR3DL1 homozygous HIV-infected individuals defined as HLA-Bw6 (a, b, c) and one uninfected KIR3DL1 homozygous individual co-expressing HLA-Bw4 were incubated with overlapping peptides from CMV pp65 (a, c) or HIV Gag (b) as described in the methods. Freshly-isolated PBMC from an uninfected KIR3DL1 homozygous individual who co-expressed HLA-Bw4 were incubated with P815 cells and anti-CD3 as described in the methods section (d). Intracellular IFN-γ production was assessed by flow cytometry with gating on CD3+CD8+ (a, b, c) or CD8+ lymphocytes (d). Unstimulated controls, including PBMC incubated with P815 cells without anti-CD3 (d) are shown in the left hand panel of each set.