A) Examination of smallest difference in dielectrophoretic mobility (ΔμDEP.min) that is calculated to be resolvable using experimentally common values of electric field strength and gradient. Absolute values (blue line, left axis) and the ratio of the minimum resolvable value divided by the nominal dielectrophoretic mobility (red line, right axis) are shown. Arrows emphasize axis associated with each plot line. Note the smallest absolute value is about 10−23 m4/V2s and relative values of about 1:104 can be separated. B) Examination of smallest difference in dielectrophoretic mobility (ΔμDEP.min) that is calculated to be resolvable using maximum experimentally accessible values of electric field strength and gradient. Absolute values (blue line, left axis) and the ratio of the minimum resolvable value divided by the nominal dielectrophoretic mobility (red line, right axis—note: logarithmic) are shown. Arrows emphasize the axis associated with each plot line. Note the smallest absolute value is about 10−26 m4/V2s and about 1:107 can be separated. C) Plots of two of the most influential factors defining the minimum resolvable physical properties of particles via g-iDEP: average dielectrophoretic mobility (μDEP,ave) and diffusion coefficient (Ddiff). Arrows emphasize the axis associated with each plot line. Note the diffusion coefficient becomes quite large at small particle diameters and the dielectrophoretic mobility becomes larger with increasing diameter.