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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Arch Biochem Biophys. 2015 May 23;579:1–7. doi: 10.1016/j.abb.2015.05.005

Figure 5. Assessing the isomerase activity of Mia40 using sRNase.

Figure 5

Panel A: mixtures containing 50 µM scrambled RNase in 50 mM phosphate buffer, pH 7.5 containing 3 mM GSH and 0.3 mM GSSG, were incubated at 25 °C with 1 µM PDI (black circles), without (grey), with 2 µM Mia40 (red), 10 µM Mia40 (blue), 2 µM Mia40APA(purple) or 10 µM Mia40APA (green circles). Aliquots were withdrawn and measured for RNase activity (see Methods). The average activity of 3 independent reactions is plotted in Panel A and the values recorded after 2 h are summarized in Panel B.