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. 2015 May 8;70(8):2191–2198. doi: 10.1093/jac/dkv119

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© The Author 2015. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Transduction of resistance genes using phages isolated from nasal swabs. Phages 80α (control), Φ19 and Φ20 transduced erythromycin resistance genes from donors KSermB or 19A [erm(C)] to recipients RN4220 or 19B. If no exogenous phages were added, no transduction was detected. Φ20 transduced resistance at a lower frequency than both 80α and Φ19 when strains were grown on 19A and resistance transferred to RN4220 (t-test, P < 0.001 and P < 0.01, respectively). 80α transduced resistance at a higher frequency than Φ19 and Φ20 when strains were grown on 19A and resistance transferred to 19B (t-test, P < 0.001). Transductant cells/mL of phage lysate are expressed as means of at least three different experiments with three replicates ± SD.