Table 1.

Genes identified by ResFinder in A. pleuropneumoniae isolates from the UK with resistance to trimethoprim and sulfisoxazole

MIDG number	Year	Location	Serovar	Trimethoprim (mg/L)	Sulfisoxazole (mg/L)	dfrA14a		sul2b		strAc		strBd
						contig	length	contig	length	contig	length	contig	length
2356	1998	Bury St Edmunds	7	>32	>512	6	3451	6	3451	6	3451	6	3451
2657	2005	Winchester	8	>32	>512	31	1757	26	943	31	1757
2664	2005	Bury St Edmunds	8	>32	>512	65	1421	55	943
3346	2005	Thirsk	8	>32	>512	48	1757	57	943	48	1757
3201	2006	Bury St Edmunds	8	>32	>512	10	1765	28	951	10	1765
3221	2006	Bristol	8	>32	256	20	1761	54	947	20	1761
3349	2006	Thirsk	8	>32	>512	47	1421	60	943
3224	2007	Bury St Edmunds	8	>32	>512	49	3429	49	3429	49	3429	49	3429
3232	2007	Thirsk	8	>32	>512	12	1761	20	947	12	1761
3370	2009	Thirsk	8	>32	>512	57	1759	106	945	57	1759
3371	2009	Thirsk	8	>32	>512	50	1759	95	945	50	1759
3372	2009	Thirsk	8	>32	>512	45	1759	102	945	45	1759
3378	2009	Bury St Edmunds	8	>32	>512	56	1753	15	4128	56	1753
3388	2009	Thirsk	8	>32	>512	6	1777	22	963	6	1777
3389	2009	Thirsk	8	>32	>512	30	1610	16	961	30	1610
3395	2010	Thirsk	8	>32	>512	5	636	26	963	74	442

^a99.8% identity (483/483 bp) with dfrA14 from Salmonella enterica subsp. enterica serovar Typhimurium (DQ388123).

^b100% identity (816/816 bp) with sul2 from Acinetobacter bereziniae (GQ421466).

^c100% identity (529/804 bp) with strA from a Shigella flexneri plasmid (AF321551) for MIDG2356 and MIDG3224, and 99.8% identity (529/804 bp) with strA from an Erwinia amylovora plasmid (M96392) for all others with strA (NB: in MIDG3395 only 512/804 bp of the gene were detected).

^d99.9% identity (705/837 bp) with strB from an Erwinia amylovora plasmid (M96392).