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. Author manuscript; available in PMC: 2015 Jul 13.
Published in final edited form as: Eur J Immunol. 2013 Mar 20;43(5):1252–1263. doi: 10.1002/eji.201242940

Figure 1.

Figure 1

Construction and characterization of MCMV-GFP-SL8. A) Schematic of recombinant strain MCMV-GFP-SL8 in which the SIINFEKL peptide was fused to GFP and cloned into the IE2 region of the MCMV genome. B) The growth of MCMV-GFP-SL8 was compared to the parental wild-type BAC-derived MCMV. Error bars represent the standard deviation from 2 to 3 plaque assays. Data is representative of 2 independent assays. C) CD8+ T cell responses for immunodominant peptides were measured in MCMV-GFP-SL8 and MCMV-WT infected mice at the indicated weeks post infection. Peripheral blood CD8+ T cells were analyzed by ex vivo intracellular cytokine staining for IFN-γ at indicated days post infection (n=3 mice per group).