Table 1.

Summary of RNA-sequencing output and read mapping analysis.

Sample name	Species	Season	Organ	SRA IDs		Total high quality reads	Library proportion
							rRNA readsa		Genlisea readsb		Non-host mRNA readsc
				Experiment	Sample		Read number	% of reads	Read number	% of reads	Read number	% of reads
NIG_SS_t	G. nigrocaulis	Summer	Trap	ERX272583	ERS257172	55,570,966	122,734 (218,975)	0.22 (0.39)	18,190,523	32.73	6,790,717	12.22
NIG_SS_l	G. nigrocaulis	Summer	Leaf	ERX272581	ERS257171	68,905,010	290,521 (492,631)	0.42 (0.71)	39,416,889	57.20	148,530	0.22
NIG_WS_t	G. nigrocaulis	Winter	Trap	ERX272584	ERS257172	39,914,128	195,871 (297,182)	0.49 (0.74)	17,107,634	42.86	2,811,952	7.05
NIG_WS_l	G. nigrocaulis	Winter	Leaf	ERX272582	ERS257171	31,504,414	127,178 (194,034)	0.40 (0.62)	21,144,415	67.12	10,564	0.03
HIS_SS_t	G. hispidula	Summer	Trap	ERX272589	ERS257479	83,721,886	131,033 (203,470)	0.16 (0.24)	13,312,311	15.90	40,547	0.05
HIS_SS_l	G. hispidula	Summer	Leaf	ERX272587	ERS257478	73,706,164	41,958 (72,126)	0.06 (0.1)	12,170,713	16.51	17,254	0.02
HIS_WS_t	G. hispidula	Winter	Trap	ERX272590	ERS257479	60,971,788	91,577 (133,429)	0.15 (0.22)	8,733,316	14.32	2,783	0.005
HIS_WS_l	G. hispidula	Winter	Leaf	ERX272588	ERS257478	60,849,726	80,315 (124,529)	0.13 (0.20)	7,471,549	12.28	538,121	0.88

^aSILVA LSURef_115 and SSURef_NR99_115 sequences were used as reference for read mapping with minimal 97% similarity (or minimal 80% similarity in brackets).

^bAnnotated G. nigrocaulis genome sequences were used as reference for read mapping with minimal 80% similarity (the G. nigrocaulis genome is 18 times smaller and has one third of the gene number compared to G. hispidula).

^cNon-redudant and trap-specific de novo assembled contigs (≥1 kbp) of G. nigrocaulis trap libraries after filtering out rRNA or Genlisea gene containing contigs were used as reference for read mapping with at least 80% similarity.