Table 1.
Sample name | Species | Season | Organ | SRA IDs | Total high quality reads | Library proportion | ||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
rRNA readsa | Genlisea readsb | Non-host mRNA readsc | ||||||||||
Experiment | Sample | Read number | % of reads | Read number | % of reads | Read number | % of reads | |||||
NIG_SS_t | G. nigrocaulis | Summer | Trap | ERX272583 | ERS257172 | 55,570,966 | 122,734 (218,975) | 0.22 (0.39) | 18,190,523 | 32.73 | 6,790,717 | 12.22 |
NIG_SS_l | G. nigrocaulis | Summer | Leaf | ERX272581 | ERS257171 | 68,905,010 | 290,521 (492,631) | 0.42 (0.71) | 39,416,889 | 57.20 | 148,530 | 0.22 |
NIG_WS_t | G. nigrocaulis | Winter | Trap | ERX272584 | ERS257172 | 39,914,128 | 195,871 (297,182) | 0.49 (0.74) | 17,107,634 | 42.86 | 2,811,952 | 7.05 |
NIG_WS_l | G. nigrocaulis | Winter | Leaf | ERX272582 | ERS257171 | 31,504,414 | 127,178 (194,034) | 0.40 (0.62) | 21,144,415 | 67.12 | 10,564 | 0.03 |
HIS_SS_t | G. hispidula | Summer | Trap | ERX272589 | ERS257479 | 83,721,886 | 131,033 (203,470) | 0.16 (0.24) | 13,312,311 | 15.90 | 40,547 | 0.05 |
HIS_SS_l | G. hispidula | Summer | Leaf | ERX272587 | ERS257478 | 73,706,164 | 41,958 (72,126) | 0.06 (0.1) | 12,170,713 | 16.51 | 17,254 | 0.02 |
HIS_WS_t | G. hispidula | Winter | Trap | ERX272590 | ERS257479 | 60,971,788 | 91,577 (133,429) | 0.15 (0.22) | 8,733,316 | 14.32 | 2,783 | 0.005 |
HIS_WS_l | G. hispidula | Winter | Leaf | ERX272588 | ERS257478 | 60,849,726 | 80,315 (124,529) | 0.13 (0.20) | 7,471,549 | 12.28 | 538,121 | 0.88 |
SILVA LSURef_115 and SSURef_NR99_115 sequences were used as reference for read mapping with minimal 97% similarity (or minimal 80% similarity in brackets).
Annotated G. nigrocaulis genome sequences were used as reference for read mapping with minimal 80% similarity (the G. nigrocaulis genome is 18 times smaller and has one third of the gene number compared to G. hispidula).
Non-redudant and trap-specific de novo assembled contigs (≥1 kbp) of G. nigrocaulis trap libraries after filtering out rRNA or Genlisea gene containing contigs were used as reference for read mapping with at least 80% similarity.