FIGURE 4.

Isothermal titration calorimetric analysis of FMN binding to FRedA/B. For ITC, measurement of heat changes (upper) and integrated peak areas (lower) of a series of 5 μl injections of 1 mM FMN into a 40 μM protein solution prepared in 10 mM Tris pH 6.0, 150 mM NaCl are shown. Experiments were carried out at 25°C.