FIGURE 5.

Effect of monomer preincubation with FMN and LjPAS on heterodimer NADH oxidase activity. Reaction mixtures contained: 40 μM FMN, 400 μM NADH, and 1 μg/mL each monomer in 100 mM MES buffer pH 5.5. Proteins were preincubated with FMN for 5 min as indicated in Row 1 before addition to the components included with NADH in Row 2. Reactions were continuously monitored at 340 nm. A = FRedA, B = FRedB, PAS = LjPAS. All reactions were performed in triplicate. Activities represent means ± standard deviations with ^∗p-value < 0.05, ^∗∗p-value < 0.01, and ^∗∗∗p-value < 0.005.