FIGURE 6.

Lactobacillus johnsonii N6.2 FRedA and FRedB interact in a bacterial two-hybrid system. Transcription activation stimulated by the interaction between FRedA and FRedB from L. johnsonii N6.2 was investigated using plasmid constructs pBR-FRedA and pACTR-FRedB in the E. coli LacZ reporter strain KDZifDΔZ. Cells were grown under static (white) or aerobic (gray) conditions, and AUs of β-galactosidase activity were determined following the hydrolysis of the CPRG substrate. All values were normalized for culture growth (OD₆₀₀). All assays were performed in triplicate. β-galactosidase activities are reported as mean ± standard deviation with ^∗p-value < 0.005 and ^∗∗p-value < 0.001.