Table 1.
Strains and plasmids used in this study.
| Strains | Description | Source |
|---|---|---|
| Lactobacillus johnsonii N6.2 | Wild type strain isolated from BioBreeding Diabetes Resistant Rats | Lai et al. (2009) |
| L. johnsonii NCC533 | Strain from Nestle Culture Collection | Hertzberger et al. (2014) |
| L. johnsonii NCC9359 | ΔLJ_0548 ΔLJ_0549 | Hertzberger et al. (2014) |
| HT-29 epithelial cells | Homo sapiens colorectal adenocarcinoma | ATCC |
| Escherichia coli strains | ||
| DH5α | F– Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 phoA supE44 – thi-1 gyrA96 relA1 | Invitrogen |
| BL21-Rosetta (DE3) | F– ompT hsdSB(rB – mB –) gal dcm (DE3) pRARE | Novagen |
| JM109 | e14– recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 D(lac-proAB) [F traD36 proABlacIqZDM15] | Stratagene |
| KDZifΔZ | [F’lacproA+,B+(lacIq lacPL8)/araD(gpt-lac)5 (DspoS3::cat)]; Kmr | Charity et al. (2009) |
| Lj-FRedA | BL21-Rosetta (DE3) carrying p15TV-fredA; Ampr | This work |
| Lj-FRedB | BL21-Rosetta (DE3) carrying p15TV-fredB; Ampr | This work |
| Lj-LjPAS | BL21-Rosetta (DE3) carrying p15TV-ljpas; Ampr | This work |
| Coex-FRedA/LjPAS | BL21-Rosetta (DE3) carrying p15TV-fredA and pCDF-ljpas; Ampr, Strr | This work |
| Coex-LjPAS/FRedA | BL21-Rosetta (DE3) carrying p15TV-ljpas and pCDF-fredA; Ampr, Strr | This work |
| Copur-FRedA/FRedB | BL21-Rosetta (DE3) carrying p15TV-fredA and pCDF-his-fredB; Ampr, Strr | This work |
| RV-1 | KDZifΔZ carrying pACTR-AP-Zif and pBRGP-ω; Kmr, Ampr, Tetr | This work |
| RV-2 | KDZifΔZ carrying pBR-fredA and pACTR-AP-Zif; Kmr, Ampr, Tetr | This work |
| RV-3 | KDZifΔZ carrying pBRGP-ω and pACTR-fredB; Kmr, Ampr, Tetr | This work |
| RV-4 | KDZifΔZ carrying pBR-fredA and pACTR-fredB; Kmr, Ampr, Tetr | This work |
| Plasmids | Description | Source |
| p15TV-L | Expression vector for protein purification, GenBank accession: EF456736; Ampr | SGC, Toronto |
| p15TV-fredA | p15TV-L carrying gene encoding FRedA from L. johnsonii N6.2 | This work |
| p15TV-fredB | p15TV-L carrying gene encoding FRedB from L. johnsonii N6.2 | This work |
| p15TV-ljpas | p15TV-L carrying gene encoding LjPAS from L. johnsonii N6.2 | This work |
| pCDF-1b | Expression vector for protein expression; Strr | Novagen |
| pCDF-ljpas | pCDF-1b carrying gene encoding LjPAS in NcoI and NotI (excludes His6 tag) | This work |
| pCDF-fredA | pCDF-1b carrying gene encoding FRedA in NcoI and NotI (excludes His6 tag) | This work |
| pCDF-his-fredB | pCDF-1b carrying gene encoding FRedB in BamHI and NotI (N-terminal His6 tag) | This work |
| pACTR-AP-Zif | Plasmid for N-terminal Zif protein fusion; Tetr | Charity et al. (2009) |
| pBRGP-ω | Plasmid for N-terminal fusions to ω subunit of E. coli RNA polymerase; Carr, Ampr | Charity et al. (2009) |
| pACTR-fredB | pACTR-AP-Zif carrying a gene encoding FRedB from L. johnsonii N6.2 | This work |
| pBR-fredA | pBRGP-ω carrying a gene from L. johnsonii N6.2 | This work |