Table 2.
Oligonucleotides used in this study.
| Protein purification | Sequence 5′–3′ |
|---|---|
| P15TV-L FRedA-F | tgtatttccagggcatgaaattactagcaattgttgg |
| P15TV-L FRedA-R | caagcttcgtcatcattatttttgagcttgttcaacacaaa |
| P15TV-L FRedB-F | tgtatttccagggcatgaaactctttgccattgt |
| P15TV-L FRedB-R | caagcttcgtcatcactacttacttacttcagcataa |
| P15TV-L LjPAS-F | ttgtatttccagggcatggcagaacctaaatggtta |
| P15TV-L LjPAS-R | caagcttcgtcatcattaatactctgaagcaccaga |
| FRedA pCDF BamHI-Fw | cgcggatcccatgaaattactagcaattgttg |
| FRedA pCDF NotIRv | atatcaatatgcggccgcattatttttgagcttgttcaaca |
| FRedB pCDF BamHI-Fw | cgcggatcccatgaaactctttgccattgta |
| FRedB pCDF NotIRv | atatcaatatgcggccgcactacttacttacttcagcat |
| Coexpression | |
| FRedB pCDF NcoI-Fw | gacatgccatggaactctttgccattgt |
| FRedB pCDF NotI-Rv | atatcaatatgcggccgcactacttacttacttcagcat |
| FRedA pCDF NcoI-Fw | gacatgccatggaattactagcaattgttg |
| FRedA pCDF NotI-Rv | tatatgcggccgctttttgagcttgttcaacaaat |
| LjPAS pCDF NcoI-Fw | gacatgccatggtggcagaacctaaatggtt |
| LjPAS pCDF NotI-Rv | tatatgcggccgcatactctgaagcaccagaa |
| Dual hybrid | |
| FRedA NdeI-Fw | ggaattccatatgatgaaattactagcaattgttg |
| FRedA NotI Rv | tatatgcggccgctttttgagcttgttcaacaaat |
| FRedB NdeI-Fw | ggaattccatatgatgaaactctttgccattgta |
| FRedB NotI Rv | tatatgcggccgccttacttacttcagcataaaaa |
| qRT-PCR | |
| Alcohol/acetylaldehyde dehydrogenase-F | gtggtcatactgcgggagtt |
| Alcohol/acetylaldehyde dehydrogenase-R | acgacatgcatccattttca |
| Cytochrome d oxidase 1-F | cactggtgagctctggttca |
| Cytochrome d oxidase 1-R | actccgccaagagttgagaa |
| Cytochrome d oxidase 2-F | tcgctactgatccagcacac |
| Cytochrome d oxidase 2-R | agctaaagcgattggcaaaa |
| Fructose/mannose inducible IIC-F | ttggtgcacaaggtgtcact |
| Fructose/mannose inducible IIC-R | tggaagcatagcaccaacag |
| PAS 10 containing protein (LjPAS)-F | atacaatgggcgctgttcat |
| PAS 10 containing protein (LjPAS)-R | acgggcattttcacttcatc |
| Lactate dehydrogenase-F | ttcaagctgctcgcgtacta |
| Lactate dehydrogenase-R | gtaagtcgcgcttagccatc |
| Surface protein Rib-F | aacacagccaattcacacca |
| Surface protein Rib-R | gctggcatttcatccttgtt |
| FtsZ cell division protein-F | agctgctgaagagagccaac |
| FtsZ cell division protein-R | tgcaattacaggagcagcac |
| Glutamine synthetase type I-F | tcggcgtgatattgttgaaa |
| Glutamine synthetase type I-R | cacctacttcgtggtgagca |
| Mannose/fructose/sorbose family IIC-F | tttacctggggattttcacg |
| Mannose/fructose/sorbose family IIC-R | aacccatcagtcaaccaagc |
| MocA family oxidoreductases-F | gcgagcaaaacagcctaaac |
| MocA family oxidoreductases-R | cttcatgggcacttgcagta |
| Hypothetical adhesin-F | taattctggtgcagctggtg |
| qRT-PCR | Sequence 5′–3′ |
| Hypothetical adhesin-R | agcggctgcatcgttaatac |
| Iron–sulfur cluster protein SufB-F | gctcgcggtactatgctttc |
| Iron–sulfur cluster protein SufB-R | tccagctccagaatcttggt |
| Manganese transporter MntH-F | agaagccctggtatcctgct |
| Manganese transporter MntH-R | gagatggcaatgcagacaaa |
| Sigma factor gene rpoD-F | ctggagttggttctcttccca |
| Sigma factor gene rpoD-R | taacatgggtttgatgaaggct |
Underlines indicate restriction sites.
Italics indicate infusion recombination sequences.