Table 1.

Number of sequence reads obtained from varying DNA input amounts

DNA amount	mtDNA copy number	Number of 454 reads
		Hypervariable region I			Hypervariable region II
		forward	reverse	total	forward	reverse	total
1 ng	~ 1 000 000	56	116	172	189	110	299
500 pg	500000	43	103	146	174	94	268
100 pg	100000	61	122	183	161	97	258
50 pg	50000	63	145	208	177	105	282
10 pg	10000	56	88	144	130	105	235
5 pg	5000	91	156	247	138	121	259
1 pg	1000	56	130	186	104	78	182
0.5 pg	500	80	131	211	195	115	310
0.1 pg	100	19	37	56	66	45	111
0.05 pg	50	24	48	72	59	52	111
0.01 pg	10	0	5	5	5	0	5
0.005 pg	5	0	4	4	4	0	4
0.001 pg	1	3	0	3	6	4	10