Table 1. No IGF1 secretion occurred in vimentin-treated cortical neurons.
| Treatment | Time after treatment | O.D. |
IGF1 (pg/ml) |
||
|---|---|---|---|---|---|
| Mean | S.E.M. | Mean | S.E.M. | ||
| Blank | – | 0.04618 | 0.00116 | ||
| Medium | – | 0.04944 | 0.00311 | N.D. | N.D. |
| Vehicle | 5 min | 0.04626 | 0.00269 | N.D. | N.D. |
| 10 min | 0.04394 | 0.00116 | N.D. | N.D. | |
| 30 min | 0.04518 | 0.00141 | N.D. | N.D. | |
| Vimentin (100 ng/ml) | 5 min | 0.04620 | 0.00189 | N.D. | N.D. |
| 10 min | 0.04456 | 0.00114 | N.D. | N.D. | |
| 30 min | 0.04164 | 0.00088 | N.D. | N.D. | |
IGF1 concentrations in the conditioned medium were measured by ELISA. IGF1 concentrations in media were calculated using a standard curve (n = 5 per group). N.D. indicates not detectable.