Table 2. No IGF2 secretion occurred in vimentin-treated cortical neurons.
| Treatment | Time after treatment |
O.D. |
IGF2 (ng/ml) |
||
|---|---|---|---|---|---|
| Mean | S.E. | Mean | S.E. | ||
| Blank | – | 0.10358 | 0.00077 | ||
| Medium | – | −0.00191 | 0.00112 | N.D. | N.D. |
| Vehicle | 5 min | 0.00742 | 0.00049 | N.D. | N.D. |
| 10 min | 0.00532 | 0.00423 | N.D. | N.D. | |
| 30 min | 0.00059 | 0.00063 | N.D. | N.D. | |
| Vimentin (100 ng/ml) | 5 min | 0.01042 | 0.00066 | N.D. | N.D. |
| 10 min | 0.00322 | 0.00131 | N.D. | N.D. | |
| 30 min | 0.00477 | 0.00380 | N.D. | N.D. | |
IGF2 concentrations in the conditioned medium were measured by ELISA. IGF2 concentrations in media were calculated using a standard curve (n = 4 per group). N.D. indicates not detectable.