Inertial focusing behavior in select channel geometries, terminating in expansions, is visualized schematically and by long exposure fluorescent imaging. Focusing performance was observed in channel expansions following: a) a horizontal (h/w < 1) high aspect ratio channel, b) an asymmetrically curved microchannel and c) a vertical (h/w > 1) high aspect ratio channel. d) Collage of long exposure particle streak images overlaying (a)–(c) at positions corresponding to increasing expansion channel width, WC1, WC2 and WC3. e) One dimensional intensity profile of long-exposure particle streak images for (a)–(c). f) Width of particle streak in the channel expansion at positions WC1, WC2 and WC3. g) Percent of channel area occupied by particles for each geometry shown in (e). Particle solutions with a concentration of 2×106 beads/mL were flowed through channels at a constant Rep=1.5.