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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Cell Mol Bioeng. 2014 Sep 1;7(3):320–333. doi: 10.1007/s12195-014-0336-9

Figure 5. Latent infections in cultured primary memory CD4+ TCM cells indicated patient-specific requirements for co-drugging.

Figure 5

A-B) Latent HIV activation induced by A) SAHA alone and B) prostratin in the presence of increasing doses of SAHA (0, 0.25, and 1 uM) in primary cultured TCM cells derived from PBMCs from 3 healthy donors. Percent of infected cells showing activation was measured by intracellular staining for p24-Gag antigen and analyzed by flow cytometry. Results were normalized to the maximal activation measured following CD3/CD28 stimulation and are presented as the mean ± standard deviation for 3 biological replicates (Donors 1 and 2) or a single replicate (Donor 3). C) Calculation of prostratin-SAHA synergy across 3 donors. Results from two independent latent infections are shown for Donors 1 and 2 (compare closed and open circles). Dotted line indicates synergy = 1 (i.e., no drug interactions).