Table 2.
Primers used to performed the amplification of the VNTRs regions of F. psychrophilum included in this study
| Primer name | Sequence (5’. > 3') | Tm°C | Target | Amplicon size (bp) |
|---|---|---|---|---|
| FpVNTR1/F | GATGGCACGAATGTCGTCGC | 57 | VNTR no 1 | 428 |
| FpVNTR1/R | TGGTTTCGGAGCATCGCCTC | 57 | ||
| FpVNTR2/F | CATGGCAACACCTTGCGCTC | 57 | VNTR no 2 | 631 |
| FpVNTR2/R | TCCCCAATACCGTTTGGCGT | 56 | ||
| FpVNTR3/F | GGCCTGGGCTATTCGTTGCA | 57 | VNTR no 3 | 320 |
| FpVNTR3/R | CCCATTGCCGAAACTACGAGC | 56 | ||
| FpVNTR4/F | GCCATCGGGGAAAACAGAGC | 57 | VNTR no 4 | 412 |
| FpVNTR4/R | TGCAACCGTACCAACAGGCA | 56 | ||
| FpVNTR5/F | CACAACCGAATTGCACGCCA | 57 | VNTR no 5 | 546 |
| FpVNTR5/R | TCGGCTGGACAAGCGCTTTT | 57 | ||
| FpVNTR6/F | TAACGCCGCTGCTTGTGCTA | 57 | VNTR no 6 | 685 |
| FpVNTR6/R | TGGCTTCTTACGCTGGTATGCA | 56 | ||
| FpVNTR7/F | TGCAACGCAGATGACACGGA | 57 | VNTR no 7 | 580 |
| FpVNTR7/R | AGGAGTAAGGCTTCTTCCCCGT | 57 | ||
| FpVNTR8/F | TGCTCTTTCGCCATAGCGGT | 56 | VNTR no 8 | 737 |
| FpVNTR8/R | CCAACGGCAAATGCTCCCAT | 56 | ||
| FpVNTR9/F | CCCGTAAATAAAACTCACAGACAG | 52 | VNTR no 9 | 670 |
| FpVNTR9/R | TGGTGCTATCCCGCCAAATG | 55 | ||
| FpVNTR10/F | ACTCCATTTGAGCAGCTGCC | 55 | VNTR no 10 | 417 |
| FpVNTR10/R | TTCGCTTACGGTATGGGAGCG | 57 | ||
| FpVNTR12/F | GCAAACGGCAATGGCCACAT | 57 | VNTR no 12 | 445 |
| FpVNTR12/R | GCTCGCGTTCCTTTCTCGGT | 57 | ||
| FpVNTR13/F | GCCCAATGGCTCAAATCGTCG | 57 | VNTR no 13 | 378 |
| FpVNTR13/R | CGCATGACCTCATCTCGGGT | 56 | ||
| FpVNTR7_2/F | TGCCAATGCGGGTGAAAAG | 57 | VNTR no 7 | 1140 |
| FpVNTR7_2/R | TCGGCACCCAATTGCAATCCT | 57 | ||
| FpVNTR13_2/F | CCAACTTGGGTTCAGGTTGTGAA | 58 | VNTR no 13 | 895 |
| FpVNTR13_2/R | ACACCAAACTACCCCGAAATGGA | 58 |
The last to set of primers were used for a few isolates analysis