Fig. 2.

Differential gene expression between unfed and fed cat fleas (Ctenocephalides felis). cDNA from whole fleas, flea gut or flea carcass harvested before, or 24 h after a blood meal were assessed by semi-quantitative RT-PCR. Beta-actin and 18S RNA were amplified as controls for the amount of cDNA used in each reaction. cDNA from bovine blood used for flea feeding and a no-template reaction were included as negative controls. The results shown are representative of two independent experiments