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. 2015 Jul 14;14:103. doi: 10.1186/s12934-015-0293-6

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© Vogl et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Type of tag and position (N/C-terminal) strongly affect volumetric HRP activities. Volumetric HRP activities in the supernatant with ABTS as substrate after cultivation on methanol for 72 h are shown. The pPpRSFC plasmids used are indicated via the numbers given in Table 1, the use of a signal sequence (MFalpha), different promoter than pAOX1 and if applicable tag and position (N/C) are given. Mean values and standard deviations of biological triplicates are shown.