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. 2015 Jul 11;8:363. doi: 10.1186/s13071-015-0964-5

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© Martins et al. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Diagnostic performance of LiHyV protein. ELISA assays were performed using sera samples of dogs with visceral leishmaniasis (n = 16), non-infected dogs living in an endemic area of leishmaniasis (n = 20), Leish-Tec®-vaccinated animals (n = 10) and animals experimentally infected by Trypanosoma cruzi (n = 13), Ehrlichia canis (n = 7) or Babesia canis (n = 7). The rLiHyV (a), SLA L. infantum (b), and rA2 (c) were used as antigens. The optical density (O.D.) values of each individual serum are shown. The cut-off values (dotted line) for negative and positive sample discrimination were calculated using the mean ± three times the standard deviation of all negative samples. The sensitivity and specificity values of the antigens were calculated and are also showed (d)