Figure 1.

White gene TALEN design and mutagenesis. (A) The white gene locus on X chromosome and design of white TALEN pair 1 (w1 TALEN) and pair 2 (w2 TALEN). w1 TALEN targets the junction between exon 5 (yellow) and intron 5 (green). w2 TALEN targets the junction between intron 4 and exon 5. w2 TALEN arms (left and right) are represented as simplified RVD repeats: each colored oval representing a particular RVD recognizing a given nucleotide, the DNA recognition sequences are highlighted in matching color, and the conserved splicing acceptor dinucleotide 5′-AG-3′ sequence is highlighted in magenta. (B) Nature of mutations introduced by w2 TALEN. Among the 10 independent single G1 flies, 9 showed short deletion mutations of varying lengths (7-, 10-, and 11-nucleotide deletions). One G1 fly showed a short insertion of 16 nucleotides (pink).