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HK-2 activity levels in a two-dimensional ovarian cancer cell (SKOV-3) culture and in three-dimensional spheroids formed on days 3 and 5 normalized to total protein content in each sample.
Notes: The values are reported as the mean ± standard deviation calculated for n=3. ****P<0.0001, one-way analysis of variance followed by Dunnett’s multiple comparisons t-test (a day 3 normoxia sample was used as the control).
Abbreviations: HK2, hexokinase-2; min, minutes.
