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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Mar Biotechnol (NY). 2014 Aug 13;17(1):81–98. doi: 10.1007/s10126-014-9595-7

Table 3.

The abundance and cysteine framework of conopeptides in Trinity (T), Newbler (N) and Combined (T+N) conopeptide datasets. For each superfamily, the conopeptide sequences of Newbler and Trinity conopeptide datasets were pooled and unique conopeptides were represented in ‘Combined conopeptide dataset’. Superfamilies R, W and Y2 were identified in Lavergne et al. (2013).

Superfamily Cysteine framework No. of conopeptides Remarks
N T T+N
Conantokin (B1) no-Cys 0 2 2
B2 no-Cys 0 1 1
D XX 2 1 3
F no-Cys 1 1 1
H XIV# 3 1 4

  • New Cys pattern
I1 VI/VII, XI 2 2 2
I2 XI, XII, XIII# 2 3 4

  • New Cys pattern
I3 XI 0 1 1
J XIV 0 2 2
K C-C-C-CC*† 0 1 1

  • New Cys pattern
L XIV, XXIII# 2 3 4

  • New Cys pattern
M C-C†, no-Cys, C-CC-C-C-C*
XXV#, VIII#, IX, XVI, III		 7 13 18

  • New Cys pattern

  • Contained M-1 branch conotoxins
N XV, C2-CC-CC-C4*1 4 2 5

  • New Cys pattern
O1 VI/VII, XI# 2 6 6

  • New Cys pattern
O2 VI/VII, XV, XII# 6 5 11

  • New Cys pattern
O3 VI/VII 3 2 4
P IX 4 5 6
S VIII, C4-CC-C4*2 2 2 3

  • New Cys pattern
Y XVII 0 1 1
MSTLGMTLL- XIX 0 1 1
MSKLVILAVL IX 1 1 1
M---L-LTVA IX, VI/VII 0 2 2
MKFPLLFISL VI/VII, IX# 0 3 3

  • New Cys pattern
Conkunitzin C4, C8, C6-CC-C6 *3 4 4 6

  • Two consecutive kunitz-like domains containing either 4 or 6 cysteine residues
Con-ikot-ikot CC-C5-CC-C54
C-CC-C6-CC-C3*5, CC-C10*6, CC-C11*7†
CC-C11-CC-C5*8
CC-C10-CC-C5-CC-C5*9
CC-C5-CC-C5*10† 		17 5 20

  • New Cys pattern

  • Contained clades with slightly divergent signal and different inter-cysteine spacing
Conodipine C10, C12 2 5 7

  • Tandem alpha and beta chain domains

  • Inter-cysteine spacing and number of cysteine residues similar to Crassostrea gigas conodipine
Conopressin/Conophysin C-C, C3-CC-C5-CC-C211 2 3 5

  • Tandem conopressin and conophysin domains

  • Inter-cysteine spacing similar to Lymnaea stagnalis conophysin
R no-Cys 0 1 1
W no-Cys 1 1 1
Y2 XII 1 1 1
*

Cysteine frameworks that are new in the conopeptides of Conus species but has been observed in other organisms,

#

The cysteine patterns that have already been identified in other conopeptide superfamilies but they’ve not been observed in this superfamily yet,

†

likely to form inter-chain disulfide bond. 1: C-C-CC-CC-C-C-C-C, 2: C-C-C-C-CC-C-C-C-C, 3: C-C-C-C-C-C-CC-C-C-C-C-C-C, 4: CC-C-C-C-C-C-CC-C-C-C-C-C, 5: C-CC-C-C-C-C-C-C-CC-C-C-C, 6: CC-C-C-C-C-C-C-C-C-C-C, 7: CC-C-C-C-C-C-C-C-C-C-C-C, 8: CC-C-C-C-C-C-C-C-C-C-C-C-CC-C-C-C-C-C, 9: CC-C-C-C-C-C-C-C-C-C-C-CC-C-C-C-C-C-CC-C-C-C-C-C, 10: C-CC-C-C-C-C-C-CC-C-C-C-C-C, 11: C-C-C-CC-C-C-C-C-C-CC-C-C, XX: C-CC-C-CC-C-C-C-C, XV: C-C-CC-C-C-C-C, XI: C-C-CC-CC-C-C, XII: C-C-C-C-CC-C-C, XIV: C-C-C-C, XXIII: C-C-C-CC-C, III: CC-C-C-CC, XXV: C-C-C-C-CC, VIII: C-C-C-C-C-C-C-C-C-C, IX: C-C-C-C-C-C, XVI: C-C-CC, VI/VII: C-C-CC-C-C, XVII: C-C-CC-C-CC-C, XIX: C-C-C-CCC-C-C-C-C, XIII: C-C-C-CC-C-C-C, C4: C-C-C-C, C8: C-C-C-C-C-C-C-C, C10: C-C-C-C-C-C-C-C-C-C, C12: C-C-C-C-C-C-C-C-C-C-C-C, no-Cys: no cysteine residues in the mature region.