FIG. 1.

Sample work flow of image processing of a single frame acquired from a cd41-egfp transgenic larva. Green fluorescence indicates specific egfp expression in thrombocytes or autofluorescence in the yolk sac. (A) Brightfield image demonstrating the orientation of 6 days postfertilization (dpf) larvae and positioning from the anterior tip of the larvae to the beginning of the yolk sac extension. (B) Raw fluorescence image before processing shows circulating and nonmobile thrombocytes, as well as background and autofluorescence. (C) Frame postprocessing showing circulating thrombocytes. (D) ImageJ “analyze particle” visual output of thrombocyte units (TU). Color images available online at www.liebertpub.com/zeb