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. 2015 Jul 14;13:225. doi: 10.1186/s12967-015-0583-0

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© Ghonim et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Purified CD4⁺ T cells procured from spleens of OVA-sensitized WT or PARP-1^−/− mice were stimulated with anti-CD3 and anti-CD28 antibodies and then skewed into a Th1 or Th2 phenotype in the presence or absence of 5 μΜ olaparib. RNA was extracted then used to generate corresponding cDNA followed by quantitative PCR with primer sets specific for mouse gata-3, il-4, t-bet, ifn-γ, or β-actin. Data is expressed as fold change with β-actin as a reference gene. Asterisk difference from CD3/CD28-stimulated cells; p < 0.01.