FIGURE 5:

Role of P-body proteins in the maintenance of P-bodies. (A–C) HeLa cells were transfected with indicated siRNAs. After 48 h, (A) proteins were analyzed by Western blotting with indicated antibodies, and (B) P-bodies were analyzed by immunofluorescence with anti-EDC4 or DDX6 antibodies, along with antibodies directed against the silenced protein to check the silencing at the individual cell level (unpublished data). Scale bar: 10 μm. (C) P-bodies were counted in three independent experiments, and their average number per cell was plotted. **, p < 0.005. (D and E) After transfection of siLSM14A and siLSM14B, proteins were analyzed by Western blotting using indicated antibodies (D). Signals were quantified in three independent experiments and plotted (E).