FIGURE 6:

Role of P-body proteins in P-body assembly after arsenite treatment. HeLa cells were transfected with indicated siRNAs and analyzed as in Figure 5, except that cells were treated with arsenite for 30 min before fixation. P-bodies were immunostained (A) and counted (B) as in Figure 5 (all), except that LSM14A antibodies were used in place of DDX6, as this accumulated in both stress granules and P-bodies. A second quantitation restricted to P-bodies larger than 450 nm was performed to show the characteristic defect observed after silencing LSM14A (large). Scale bar: 10 μm. The corresponding Western blot analysis is presented in Supplemental Figure 2D.