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. 2015 Jul 15;26(14):2664–2672. doi: 10.1091/mbc.E14-11-1564

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© 2015 Cavolo et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — MB inhibits retrograde axonal transport of DCVs in Drosophila. (A) FRAP of ANF-GFP in the lateral td neuron soma and proximal axon is abolished by MB. Top, control incubation with vehicle (Con) images show FRAP in the soma and the proximal axon (the rest of the neuron is outside the field of view). Bottom, after 20 min in 2 μM MB, no FRAP is evident. Scale bar, 5 μm. (B) Quantification of FRAP by counting DCVs that entered the proximal axon and soma in 20 min after photobleaching; Con, n = 8; MB, n = 6. **p < 0.01. Error bars represent SEM.