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. 2015 Jul 15;26(14):2664–2672. doi: 10.1091/mbc.E14-11-1564

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© 2015 Cavolo et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Analysis of MB on dynactin integrity by sucrose density centrifugation. (A, B) HEK293T cell lysate treated with vehicle (A) or 2 μM MB (B) was subjected to sedimentation into 5–20% sucrose gradients, and gradient fractions were analyzed by immunoblotting for dynactin subunits and the dynein IC. Densitometry indicates about half of the Arp1 in a monomer/dimer pool in the presence of MB vs. <5% in the vehicle control. (C, D) Analysis of MB on dynactin integrity in vitro. Purified bovine dynactin treated with vehicle (C) or 2 μM MB (D) was subjected to sedimentation into a 5–20% sucrose gradient, and gradient fractions were analyzed by PAGE (stained with Coomassie blue).