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. 2015 Jul 15;26(14):2673–2684. doi: 10.1091/mbc.E15-04-0224

FIGURE 2:

FIGURE 2:

EDTB overexpression results in loss of growth control. (A) Representative cells expressing ET-FL or GFP-CD labeled with PHH3. Proliferation is increased with overexpression of GFP-CD or ET-FL. Error bars, SEM of percentage of cells that are PHH3 positive; ***p < 0.005, **p < 0.01. (B) GFP-CD cells form multiple small, multilayered foci (arrows) when grown on Transwell filters, whereas control cells remain as a monolayer with limited number of foci. Transmission electron microscopy (TEM) of control and GFP-CD cells grown on filters shows that control cells grow as a monolayer but GFP-CD cells form multicellular foci. Foci of GFP-CD–expressing cells maintain epithelial characteristics, such as apical microvilli (arrowheads). Scale bars, 100 μm (phase), 1 μm (TEM). (C) Quantification of the multilayer area shows an increase in foci formation in cells expressing GFP-CD. Error bars, SEM; ***p < 0.005. (D) Immunofluorescence labeling of apical (gp135, podocalyxin) and basolateral (E-cadherin) markers shows that the polarized distribution of these proteins is maintained with expression of GFP-CD. The foci (arrowheads) also maintain this polarized expression pattern. Scale bar, 20 μm.