FIGURE 6:

Increased expression of EDTB results in growth in soft agar and is associated with early-stage LIHC samples. (A) MDCK cells expressing GFP (control) or GFP-CD were grown in soft agar for 3 wk. GFP-CD cells form long, branching structures (arrows) that produce satellite colonies (arrowheads) after 3 wk in culture. Scale bars, 100 μm (left), 20 μm (right). Right, quantification of colony formation after 14 d in cells expressing GFP-CD. Error bars, SEM; **p < 0.01. (B) Using the Cancer Genome Database, we collected the rsem expression values for EDTB in 105 liver hepatocellular carcinomas. The tumors were binned into three equal groups based on EDTB expression levels. Separating these groups by tumor stage shows that 44% of stage I tumors express EDTB at high levels.