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. 2015 Jul 15;26(14):2698–2711. doi: 10.1091/mbc.E14-09-1378

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© 2015 Hellesøy and Lorens. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — Constitutive Akt1 activity blocks capillary-like network formation. HUVECs and HDMVECs expressing mutant myrAkt1 constructs were grown in coculture with PaSMCs. (A) Fluorescence microscopy images show that control cells of both cell types form interconnected branched capillary–like networks. Constitutive activation of Akt1 in ECs inhibited network formation in a grade-dependent manner. (B) Quantification of network formation confirmed that HUVECs and HDMVECs expressing hemiphosphorylated Akt1 mutants had a significantly shorter tube total length than control cells (set to 100%). (C) Networks formed by Akt1 mutant cells also had a significantly lower number of branch points than control cells. Asterisk indicates statistically significant difference from control (set to 100%). *p < 0.05, min n = 9, max n = 14.