TABLE IV. Precision, accuracy and recovery data for isoniazid (INH) quantification by high-performance liquid chromatography-tandem mass spectrometry.
| Validation parameters | INH quality control
concentration (ng/mL) |
|||
|---|---|---|---|---|
| 100 | 200 | 2,000 | 5,000 | |
| Precision (RSD) (%) | ||||
| Intra-run (n = 8) | 8.15 | 11.68 | 7.63 | 5.78 |
| Inter-run (n = 24) | 10.22 | 7.20 | 5.75 | 8.65 |
| Accuracy (%) | ||||
| Intra-run (n = 8) | 102.06 | 102.13 | 99.00 | 95.20 |
| Inter-run (n = 24) | 99.15 | 104.49 | 105.99 | 101.81 |
| Recovery (%) (n = 8) | - | 80.35 | 88.59 | 94.58 |
RSD: relative standard deviation.