TABLE II. Comparison of results from the cytochrome b-polymerase chain reaction (Cytb-PCR) and real-time PCR Plasmodium detection assays using field-caught Anopheles darlingi .

	Real-time PCR positive (n)	Real-time PCR negative (n)	Sensitivity (%)	Specificity (%)
Cytb-PCR positive	1	20	3.33	93.38
Cytb-PCR negative	29	282	-	-

Cohen’s kappa = -0.04; McNemar’s test χ² =1.653, p > 0.1.