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. 2015 May 23;7:37–40. doi: 10.1016/j.nmni.2015.05.005

Table 2.

MLVA profiles collated from six international laboratories after investigation of 30 Mycoplasma pneumoniae DNA samples

Sample Expected profilea Lab 1 Lab 2 Lab 3 Lab 4 Lab 5 Lab 6
1 (M129) 44572 44572 43562 44572 44572 44572 43562
2 (1000 copies/μL)b 43662 43662 42652 43662 43662 43662 42652
3 (100 copies/μL) 43662 43662 42652 4–662 4356 -c 43662 - 255c -
4 (10 copies/μL) 43662 43662 - - 652 4–6-2 - - - - - 43662 - - - - -
5 (1 copy/μL) 43662 - - - - - - - - - - - - 6 - - - - - - - - - - - - - - - - -
6 43662 43662 42652 43662 43662 43662 42652
7 43662 43662d 42652 43662 43662 43662 42652
8 53662 63662c 52652 53662 53662 53662 52652
9 54572 54572 53562 54572 54572 54572 53562
10 63662 63662 62652d 63662 63662 63662 62542c
11 34572 34572 33562 34572 34572 34572 33562
12 (M129) 44572 44572 43562 44572 44572 44572 43562
13 63562 63562 62552d 63562 63562 63562 62552
14 33562 33562 32552 33562 33562 33562 32552
15 63562 63562 62552 63562 63562 63562 62552
16 53662 53662 52652 53662 53662 53662 52652
17 23662 23662 22652 23662 23662 23662 22652
18 44572 44572 43562 44572 44572 44572 43562
19 23562 23562 22552 23562 23562 23562 22552
20 43572 43572 42562 43572 43572 43572 42562
21 54572 54572 53562 54572 54572 54572 53542c
22 54572 54572 53562 54572 54572 54572 53562
23 43662 43662 42652 43662 43662 43662 42652
24 34572 34572 33562 34572 34572 34572 33562
25 63562 63562 62552 63562 63562 63562 62552
26 34572 34572 33562 34572 34572 34572 33562
27 33662 33662 32652 33662 33662 33662 32652
28 23662 23662 22652 23662 23662 23662 22652
29 33662 33662 32652 33662 33662 33662 32652
30 54572 - - - - - - - - - - 54572 5–572 54572 - - 562c

Repeat number different from the expected result are underlined. A hyphen indicates no amplification.

MLVA, multiple-locus variable-number tandem repeat analysis.

a

Naming of profiles was based on the method in which naming is based on a string of allele numbers in order of MPN1, MPN13, MPN14, MPN15 and MPN16 showing the actual number of repeats at each locus.

b

Samples 2, 3, 4 and 5 are dilutions of the NTCT10119 FH commercial standard strain with 1000, 100, 10 and 1 copies/μL, respectively.

c

MLVA profile remains different from the expected MLVA profile after correction for transcription errors and interpretation differences.

d

MLVA profiles with initial transcriptional errors.