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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: J Bone Miner Res. 2014 Dec;29(12):2676–2687. doi: 10.1002/jbmr.2296

Fig. 1.

Fig. 1

GATA4 regulates ERα and E2 target genes. (A) Calvarial osteoblasts were infected with lentivirus expressing either shGFP or shGATA4. Cells were then differentiated for 2 wk and RNA was obtained. qPCR was performed for ERα and normalized to actin mRNA. (B) Calvarial osteoblasts were infected with lentivirus expressing either shGFP or shGATA4. Cells were then differentiated for 2 wk and then treated with 10 nM E2 for 24 hours. Protein was obtained and immunoblots for ERα, GATA4, and actin were performed. (C) RNA was obtained as in A, and qPCR was performed with primers to FasL and normalized to actin mRNA. (D) RNA was obtained as in A, and qPCR was performed with primers to alkaline phosphatase and normalized to actin mRNA. *p < 0.05; **p < 0.001. Alk. Phos. = alkaline phosphatase.