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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: J Immunol. 2015 May 27;195(1):217–226. doi: 10.4049/jimmunol.1402808

Figure 3. Twist1 and Twist2 cooperate with each other for enhanced binding to cytokine promoters after chronic NOD2 stimulation.

Figure 3

(A) MDMs (n=4) were left untreated or pre-treated with 100μg/ml MDP for 48h, and then treated with 100μg/ml MDP for an additional 4h (acute). (B) MDMs (n=4) were left untreated (for acute) or pre-treated with 100μg/ml MDP for 24h, then transfected with scrambled, Twist1 or Twist2 siRNA alone or in combination, and 24h later (total 48h MDP pre-treatment) MDMs were treated with 100μg/ml MDP for an additional 4h (acute). (A-B) Recruitment of Twist1 and Twist2 to cytokine gene promoters was assessed by ChIP. Fold enrichment normalized to untreated, scrambled siRNA-transfected cells + SEM. (B) Statistical significance above the knockdown sample bars is compared to its corresponding scrambled siRNA sample. *, p<0.05; **, p<0.01; ***, p<0.001. Tx, treatment.