Fig 2. The effect of fibril fragmentation on length and its capacity to cause membrane disruption.

Liposome dye release assay using a model lipid membrane formed from 80% (w/w) phosphatidylcholine and 20% (w/w) phosphatidylserine encapsulating the fluorescent probe carboxyfluorescein. Dye-encapsulated vesicles were incubated with amyloid fibril samples (30 min): (A) α-Syn 100 μg/mL (B) Lysozyme 20 μg/mL and (C) Aβ40 20 μg/mL. Fluorescence was then recorded and the fold increase in dye release was normalised against unfragmented fibril fluorescence. Light grey bars denote unfragmented fibril samples and dark grey bars denote fragmented fibril samples (n = 3, error bars show SE). Statistical significance between the unfragmented and fragmented fibril dye release response was determined by a Mann Whitney U test P ≤ 0.05 (*) or P ≤ 0.005 (**).