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. 2015 Jun 19;36(2):369–376. doi: 10.3892/ijmm.2015.2253

Figure 1.

Figure 1

Identification and expression of mature and immature Pyropia yezoensis protein 1 (PYP1) gene transcripts. (A) Schematic representation of PYP1 and its variants produced by complete and alternative splicing, respectively, compared with the genomic organization of the PYP1 gene. Empty boxes represent exons, while bars represent introns. Positions of stop codons are indicated in the schemes of each variant. AV numbers determined by the Kazusa DNA Research Institute are names of clones whose sequences were determined previously. Arrows indicate the positions of primers used for RT-PCR; (Table I): F, PYP1-F; R, PYP1-R; AC-R, PYP1-AC-R; B-R, PYP1-B-R. (B) Gel images showing the results from RT-PCR. Comparison of PYP1 gene expression between gametophytic (G) and sporophytic (S) generations (left panel). The expression pattern of PyMBF1 (19) is presented as a reference. The presence of transcripts from the mature form and splice variants in the gametophytic generation can be noted (right panel). The sizes of the RT-PCR fragments for PYP1, PYP1-AC and PYP1-B are 246, 232 and 141 bp, respectively. M, protein marker.