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. 2015 Jun 19;36(2):369–376. doi: 10.3892/ijmm.2015.2253

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Copyright © 2015, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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SDS-PAGE analysis of recombinant PYP1s expressed in the E. coli strain BL21. Samples were resolved by 17% SDS-PAGE. Recombinant proteins are indicated by an asterisk. (A) Recombinant PYP1. (a) M, protein marker; lane 1, before IPTG induction; lane 2, after IPTG induction; lane 3, insoluble protein; lane 4, first purified, soluble protein; (b) M, protein marker; lane 1, before IPTG induction; lane 2, after IPTG induction; lane 3, second purified, soluble protein. (B) Recombinant PYP1-AC. (a) M, protein marker; lane 1, before IPTG induction; lane 2, after IPTG induction; lane 3; soluble protein; lane 4, first purified, soluble protein; (b) M, protein marker; lane 1, control vector; lane 2, second purified, soluble protein. (C) Recombinant PYP1-B. (a) M, protein marker; lane 1, before IPTG induction; lane 2, after IPTG induction; lane 3, soluble protein; lane 4, insoluble protein; lane 5, first purified, soluble protein fraction 1; lane 6, first purified, soluble protein fraction 2; (b) M, protein marker; lanes 1 and 2, second purified, soluble protein. PYP1, Pyropia yezoensis protein 1.