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. 2015 Jun 8;36(2):355–362. doi: 10.3892/ijmm.2015.2238

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Copyright © 2015, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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Analysis of neuronal marker profiles in ES/Con and ES/miR-125b-2 groups. The expression levels of neuronal markers Nestin and Map2 were detected by reverse transcription quantitative polymerase chain reaction. The individual values were normalized against the endogenous standard gene GAPDH. Values are expressed as the mean ± standard error of data from one representative of three experiments, performed in triplicate. ^*P<0.05 compared to ES/Con. ES/Con, empty vector-transfected ESCs; ES/miR-125b-2, miR-125b-2-transfected ESCs; ESC, embryonic stem cell; miR, microRNA; Map, microtubule-associated protein.