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. 2015 May 29;36(2):415–423. doi: 10.3892/ijmm.2015.2229

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Copyright © 2015, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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Nicorandil exerts little effect on MSC proliferation. Various concentrations of nicorandil were added to the culture medium for the indicated periods of time in order to examine its effects on MSC viability and proliferation. (A and B) The EdU assay indicated that nicorandil had little effect on MSC proliferation, even at >1,000 μM, and did not induce cell toxicity (P>0.05). (C) Growth curves of MSCs obtained by CCK-8 assay, indicating no significant changes during the 2 days of treatment with various concentrations of nicorandil. Data are presented as the means ± SD of 3 separate experiments. Nico, nicorandil; MSCs, mesenchymal stem cells.