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PRP-1 effect on the expression of SCML2 in human JJ012 chondrosarcoma cell line. Mouse monoclonal anti-SCML2 (SCMAD14a), was used in 1:1,000 dilution, and secondary anti-mouse IgG at 1:5,000. Band was detected ~100 kDa region. Film exposure time, 2–5 min. All the antibody dilutions were made with Western Blocker solutions. Mouse monoclonal anti-tubulin antibody was used at 1:2,000 and secondary anti-mouse IgG at 1:5,000. Tubulin band was detected at 55 kDa.
